![]() ![]() While this makes the Y-axis hard to interpret visually, it provides correct units for the slope (which equals -1/Kd). While this is easy to interpret (it is the fraction of radioligand bound to receptors), an alternative is to express specific binding in sites/cell or fmol/mg protein, and to express the free radioligand concentration in nM. Some investigators express both free ligand and specific binding in cpm so the ratio bound/free is a unitless fraction.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |